Determination of km and V max of B- Amylase To determination of km and V max of B-Amylase. Theory: – B-amylase form higher plants catalyses the hydrolysis of the and 1- 4 glycoside linkage in the polysaccharides to give maltase. It is an example exoenzyme and therefore splits off maltose unit from free non reducing…
Estimation of RNA by Orcinol MethodTo estimate RNA in a given sample by orcinol method. When a solution of RNA is treated with orcinol. Reagent, it gets hydrolyzed to component base, phosphate and rebase reduce. Further heating with orcinol produces bluish green complex in presence of Fe+3. Requirement: – Std. soln of RNA [0.05 mg/ml]…
How casein is Isolated from Milk Casein is the main protein found in milk and is present at a concentration of about 3-5 gm / 100 ml. It is actually a heterogeneous mixture of phosphorus containing proteins and not a single compound.Most protein show a minimum solubility at their iso electric point and this principle…
How to determine the Vitamin C content of given fruit juice and drumstick leaves. Principle: – Chemical Assay of vitamin C depends on the marked reducing properties of the vitamin. Generally vit. C is determining in plant or animal extract by its reducing action on the dyestuff. 2, 6 dichloride phenol indophenols. In this method…
B-amylase from higher plants catalyses the hydrolysis of 1, 4 – glycosides bands to maltose. It is an examples i.e. splits maltose units from non-reducing end. When a branch point is reached, the hydrolysis ceases giving limit dertin. The name B-amylase is given because the initial product of hydrolysis is B-maltose which lutes on comes…
How to determine optimum pH of Amylase Principle: – Optimum pH of an enzyme is the PH at which the reaction velocity is maximum. Each enzyme has a characteristic PH at which it reacts at maxi, velocity. The optimum PH is determined by carrying out an enzyme reaction at various PH values.In the case of…
To study the effect of temperature on B- AmylasePrinciple: – B-Amylase catalyses the hydrolysis of B-1, 4 glycosides links in starch with the Production of reducing sugar. The amylase activity is determined in terms of amt. of reducing sugars produced at the end of the reaction. These sugars reduce 3, 5 dinictrosalieylic acid (DNS) to…
Estimation Of Glucose By Anthron method Principle: – A very useful method for the colorimetric determination of carbohydrates is based on the reaction of an throne with carbohydrates in the presence of strong H2SO4. The conc. H2SO4 hydrolyses the glycoside i.e. bonds to give monosaccharide which are then a hydrated to give furfural and its…
Estimation of vitamin by potassium iodated method Principle: – The chemical assay of vitamin C depends on the marked reducing properties of the vitamin. In this method potassium iodated in acidic condition is used as oxidizing agent. In presence of high conc. of HCI (exceeding 4N) the iodine produced us oxidized by iodated to iodine…
Principle:- Protein reacts with the Folin Go Chateau reagent to form a colored complex. The co lour is so formed due to the reaction of alkaline ca++ with protein as in the Burette method and the reduction of Phosphomolybdate[ Mo+6—–> Mo+4 ] by tyrosine and tryptophan present in protein. The intensity of co lour depends…