How to determine the DNA content of given sample by diphenylamine reaction. When DNA is treated with diphenylamine acidic conditions a blue compound is formed with sharp absorption maxima at 595 nm. This reaction is given by 2’- deoxy pentose in general and is not specific for DNA. In acid soln the straight chain form…
HOW TO ESTMATE OF LIPASE ACTIVITY Lipase is a fat splitting enzyme. It hydrolyses fats into fatty acids and glycero Trigycerides +Lipase enzyme——————->Fatty Acids +GlycerinFor lipase optimum PH is around 7-7.2 and optimum 10 mp is 370c.The enzyme unit I.O amount of enzyme that liberates fatty acid which consumes 1 ml of 0.05 m alkali…
How to estimate alfa – amino nitrogen of given sample of protein hydrolyses. This method is based on the formation of soluble complex between amino acid and cu++ ions present in the form of cupric phosphate Cu3 (PO4 )2 . The sample is treated with excess of cupric phosphate and the excess of cupric phosphate is…
How to estimate the alfa-Tocophorol content of given sample Alfa – Tacopherol occurs widely distributed in plant and animal tissue but only in trace amounts. There are 5 naturally occurring to copherols α-, β-, γ- and δ θ amongst which α- tocopherol shows maximum biological activity. It is an antistenlity vitamin. The esters of vitamin. Are more…
Protease of proteolysis enzyme cafulyzes the breakdown of protein to peptides, and amine acids. There are 3 sources of proteases 1) Plant source 2) Animal source 3) Microbial source. Try sin, chemotropism, pepsin etc. are animal protease. They occur in gastro intestinal tract of all animals’ pepsin is an acid protease which has broad specificity.…
Estimation of Blood Glucose by Folin – wu method Glucose quantitatively reduces ul baric ions to cuprous ions in a boiling alkaline solution. It gets oxidized to glycolic acid, consequently reduce phosphomolybdic acid [Mo +6 Mo +4] are themselves oxidized back to cubic ions. The reduced form phosphonolybdic acid imparts a blue co lour which…
How to estimate polyphone oxidase, activity from 1) Potatoes. 2) Apples 3) Bringal. Enzymic browning occurs in many fruits; vegetables like potatoes apples, banana. When the tissue is cut, peeled and exposed. Injured tissue rapidly dowels on exposure to air due to concession of phonemic comp to brown melanin. The enzyme that initiates this is…
Phosphataes are enzymes capable of cattily zing the hydrolysis of certain organic phosphates. The general reaction may be written as. Depending upon PH optima, they are classified on acid or alkaline phosphates. An acid phosphate has PH optima between 7.6 and 9 depending upon their origin. The principle of measurement of activity is the determination…
B-Fructofuranosidase is formed in yeast and catalyses the hydrolysis of sue rose to glucose and fructose. Enzyme is inverters of sucrose. Substrate is non reducing sugar and product formed is a reducing therefore can be easily identified. During isolation, it is necessary to avoid loss of activity due to denaturizing of enzyme. Inactivation may be…
Todays post we are providing our readers a very important piece of US FDA’S document for what types of documents are required to be submitted along with documents for Process Validation of sterilisation process , for an ANDA or INDA for Human and veternary ( pharmaceutical ) Drugs , this document is published in 1994 by US FDA .…