What is a Laminar Air Flow Cabinet.

What is a Laminar Air Flow Cabinet.

Laminar air flow cabinets can provide a clean room environment of class 100 and below, which is attained by filtering air through HEPA filters , they can remove particulate matter of dimension 0.3 nm and above with an efficiency of 99.997 to 99.999.Hence an appropriately designed and maintained laminar air flow cabinet can provide a sterile environment for handling material which are required to be protected from getting contaminated and from contaminating other material and personals and environment (to prevent cross contamination ).

Laminar air flow cabinets are used in aseptic transfers  (also see aseptic techniques ) and aseptic manufacturing process as well as to facilitate handling of microbiological preparations who’s sterility is required to be maintained, as well as to prevent cross contamination during material handling , critical activities which are likely to evolve dust particles like raw material dispensing in pharma manufacturing process are carried out in a laminar air flow cabinet.

In laminar Air flow bench air is filtered through HEPA filters and blown though towards user with a velocity of 0.45 meters/second (90 feet per minute) across the working table . To minimize the upstream particle load inlet air may be filtered through prefilters first and then blown though high efficiency HEPA filters to remove the particles completely, which results in generation of an uniform and smooth air flow pattern where a layer air flowing in one direction do not cross or diffuse in to path of other layer or in to the other layer maintain a pattern of flow like playing cards moving one over the other, such pattern of air flow is called as laminar air flow, which helps in removing the particulate mater away from the path which are then filtered away in recirculation. In other pattern where all layers flow in a zigzag manner in all directions diffusing in to each such flow of air is called as turbulent air flow, same terminology is applied in describing flow in liquids too.

Laminar air flow cabinets can be designed to provide air flow in horizontal as well as vertical air flow direction, both types has their own advantages; regulatory accepted standard for LAF is NSF49 standard.
Laminar flow cabinets are fitted with a Ultraviolet light lamp to keep the shell and content sterilized (see sterility testing), when it is not in use and ultra violet light lamp is required to switch off during use.Laminar air flow cabinets are built with stainless steel in a manner so the surfaces at all sealing points are smooth with no gaps at joints which might help in accumulation of microbial spores and then contamination.

Apart from pharmaceutical industry laminar air flow cabinets are also used in other industry where a material is required to be protected from getting contaminated with particulate matter example is computer where a small particulate matter on the disk can damage entire disk. Laminar Air flow cabinet is also termed as laminar flow bench , laminar flow closet or tissue culture hood.
Biosaftey cabinets:
Biosaftey cabinets are meant for handling hazardous pathogenic microorganisms, viruses , they differs from laminar air flow bench with respect to air circulation pattern, in laminar air flow cabinets air is filtered through HEPA and it is blown towards user and in biosaftey cabinets air is taken in over sample being handled (inside) with velocity ranging from 100 ft/min and the exhaust air is filtered through HEPA filters, to attain higher level of safety about 60% of air from the rear grille is exhausted and 40% air is recirculated. Biosaftey cabinets can be completely enclosed by providing glove attachment from front side.

Also see Aseptic Techniques followed while using Laminar Air Flow Cabinet

Do you know now this website has become a most popular and most referred website in pharmaceutical industry and in pharmaceutical companies by pharmaceutical manufacturers from all over the world for almost all topics related to FDA guildelines, GMP guidelines for Pharmaceutical Manufacturing (Current Good Manufacturing Practice for Pharmaceutical Manufacturing , c GMP guidelines ),and technical aspects of pharma manufacturing research and development , Pharma Regulatory Affairs  and latest news and new drugs developments.
You may also like following articles

What is Therapeutic Index ?

What is Narrow Therapeutic Range Drug?

What is High Efficiency Particulate Air( HEPA ) filter ?

What is 21 CFR Part 11, US FDA compliance and CFR 21 Part 11?

CFR 21 part 11 and its application on computarised systems used in clinical trials US FDA guidelines

What is High-Efficiency Particulate Air  HEPA Filter

What is 21 CFR Part 11, US FDA guidelines requirements of FDA compliance and CFR 21 Part 11.

What is a Site Master file of a pharmaceutical company

What is Generic Drug

What is Reference Listed Drug  ? ( RLD )

What is Pharmaceutical Equivalents

What is Pharmaceutical Alternatives

What is Therapeutic Equivalents

What are Post Market studies

Why a drug is bound to protein, What is protein binding?  What is drug absorption , distribution ?

Do Physical properties contribute to drug activity.

What is drug receptor , How a drug resistance occurs

Mechanism of drug resistance

What is drug interaction

Drug interaction, and its examples

What is first pass metabolism of a drug

What is What is 510(k) Clearances ?

What is 510(k) Clearances,

Premarket Notification for medical devices – PMN or 510(k)

What is a drug interaction

Examples of drug interactions

Antibiotic Definition and classification

Antibiotic Resistance and Antibiotic resistance mechanism

Antioxidants food supplements

Vitamin D Details on FDA cautions on accurate dosage of Vitamin D

What is an antibody? what is monoclonal and polyclonal antibodies?

Terminologies In vaccine Production

Multi stage testing of Virus vaccine production

Testing of vaccines at different stages of production


Enzyme linked immunosorbent assay ELISA

Raido Immuno assay


Pharmaceutical Aseptic Manufacturing Process Terms , Terminology and Definitions.


Here are some articles which will be useful for you in further understanding of aspects of sterile dosage form manufacturing and regulatory affairs and good manufacturing practice in

pharmaceutical industry

Pharmaceutical Validation

Types of validations in pharmaceutical manufacturing

Requirements of documents for validation of sterilisation process

How to investigate OOS out of specification results

Determination of Phenol coefficient of a disinfectant

Sterility testing

Clean Room Classification

Time limitations in sterile pharmaceuticals processing

Aspects of validation of manufacturing process in sterile pharmaceuticals

Clinical Trials

Requirements of US FDA Inspections of Clinical Investigators of Clinical Trials




Controlling Pyrogens in injectable dosage forms

Media fill run process simulation aspects Validation of Aseptic Process and Sterilisation

Injectable Dosage Form Asepict Techniques  Sterile dosage form manufacturing imprtant aseptic techniques

New Drug Application (NDA) how to make a New Drug Application (NDA) to US FDA

Abbreviated New Drug Application (ANDA) What is ANDA , detailed information about ANDA preparation and submission to US FDA

How to make Investigational New Drug (IND) Application to US FDA

Drug applications submission to us fda Over the counter Drugs OTC drugs


Electronic record in pharmaceutical manufacturing industry

Good manufacturing practice in pharmaceutical industry

Pharmaceutical industry pharmaceutical companies and FDA latest updates

Here is an interesting article on world wide pharmaceutical industry and making a carrier in one of pharmaceutical companies article on Pharmaceutical Industry pharmaceutical industry

Find a Job in Pharmaceutical Company –>JOBS IN PHARMA INDUSTRY<—
To Find Pharmaceutical jobs and make a Pharmaceutical careers see here pharmaceutical companies
Here are some interesting articles on Quality assurance systems for pharmaceutical company

Quality assurance in pharma industry

Quality by design concept for pharmaceutical industry

Quality by design concept in pharmaceutical industryan explanation

Useful Books For Pharmaceutical Manufacturers and Pharmaceutical Professionals
What is Therapeutic Index ?

What is Narrow Therapeutic Range Drug?

What is What is a High-Efficiency Particulate Air (HEPA) filter ?

What is 21 CFR Part 11, US FDA compliance and CFR 21 Part 11.

One comment

Leave a Reply