Estimation of RNA by Orcinol Method
To estimate RNA in a given sample by orcinol method.
When a solution of RNA is treated with orcinol. Reagent, it gets hydrolyzed to component base, phosphate and rebase reduce. Further heating with orcinol produces bluish green complex in presence of Fe+3.
Requirement: – Std. soln of RNA [0.05 mg/ml] sample solution of RNA, fresh orcinol reagent.
Procedure: – Mix 2 ml of sample and standard solution of RNA with 3 ml of orcinol reagent. Heat on boiling water bath for 30 mins. Cool and determine extinction at 665 nm. Against oreinol blank.
Calculation: – Conc. of sample = (Conc. Of standard / Absorbance of std.)x Absorbance of sample
= 0.05 /0.249* 0.448
= 0.8945 mg / ml.
The conc. of RNA in given sample was found to be 0.8945 mg/ml.
This website is a guide for pharmaceutical manufacturing and for pharmacy students and pharmaceutical regulatory affairs proffesionals and for every one associated with pharmaceutical industry , we recommend you to subscribe to this website by entering your email ID in the box provided so that you will receive our articles as and when our website is udated.
This website is most favorite Amongst proffesionals in almost all pharmaceutical companies in USA , Pharmaceutical companies in UK , Pharmaceutical companies in Japan , Canada , UAE , Pharmaceutical companies in India
Following are some of articles which;will be useful for you in further understanding of aspects of sterile dosage form manufacturing